The Enzyme Peroxidase Lab Report - 1548 Words

The Enzyme Peroxidase Case Study Lab Report Autumn Vick I. ABSTRACT: The purpose of this experiment is to learn the effects of a certain enzyme (Peroxidase) concentration, to figure out the temperature and pH effects on Peroxidase activity and the effect of an inhibitor. The procedure includes using pH5, H202, Enzyme Extract, and Guaiacol and calibrating a spectrophotometer to determine the effect of enzyme concentration. As the experiment continues, the same reagents are used with the spectrophotometer to determine the temperature and pH effects on Peroxidase activity. Lastly, to determine the effect of an inhibitor on Peroxidase, an inhibitor is added to the extract. It was found that an increase in enzyme concentration also caused an increase in the reaction rate. The reaction rate of peroxidase increases at 40oC. Peroxidase performed the best under pH5 and declined as it became more basic. The inhibitor (Hydroxy-lamine) caused a decline in the reaction rate. The significance of this experiment is to find the optimal living conditions for Peroxida se. This enzyme is vital because it gets rid of hydrogen peroxide, which is toxic to living environments. II. INTRODUCTION: There are thousands of chemical reactions that occur in a cell at every moment. These chemical reactions do not occur randomly, they are highly under the control of biological catalysts called enzymes. Most of these enzymes are proteins. These proteins have certain primary structures directed byShow MoreRelatedPeroxidase Enzyme Biology Lab Report (Half Way Finished) Essay examples635 Words   |  3 PagesHow Enzymes Work In Different Environments By Sarah Smith Biology1111 October 20, 2011 Lab Partner: Nellie Greer ABSTRACT Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, H2O2, into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this, we added different levels of pH, low, medium, and high, into different test tubes with the enzyme and H2O2,Read MoreInvestigating The Rate Of Enzyme Activity Within A Experiment Essay1179 Words   |  5 PagesThe purpose of this report is to determine which catalyst effect the rate of enzyme activity within a experiment. Enzymes lower the energy of activation by binding with substrate in chemical reactions to allow the reaction to occur. energy activation is the reguired amount of energy required amount of energy needed to start a chemical reaction. This decreases the faster the enzyme reaction goes by the shape change on an active site. the conclusion is that the enviromental conditions such as pH, temperatureRead MorePeroxidase Es say1460 Words   |  6 PagesIn a 1-2 page report, address the following. The first part of your report is the Results section of a scientific paper. In this section, present the results of your experiment without any interpretation. Be sure to adequately answer the following questions: How did the rate of the reaction change as you changed each of the three variables? If you vary the temperature, after a certain temperature, the enzymes will become denatured and then the rate will decrease. Each enzyme functions atRead MoreEssay on Horseradish Peroxidase1933 Words   |  8 Pagesboiling and concentration on Horseradish Peroxidase ABSTRACT The purpose of this report is to find out the effect of change in the Temperature, PH, boiling, concentration in peroxidase activity. Peroxidase is an enzyme that converts toxic hydrogen peroxide (H2O2) into water and another harmless compound. In this experiment we use, turnips and horseradish roots which are rich in the peroxidase to study the activity of this enzyme. The activity of peroxidase with change in temperature was highestRead MoreElisa: Immune System and Serum D= Donor Essay665 Words   |  3 Pages      Lab Report: 10 ELISA       Purpose: This test is often used to see if you have been exposed to viruses or other infectious substances. It is frequently used to screen for present or past infections.       Introduction: ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An ELISA or enzyme-linked immunosorbent assay is a method used in the laboratory to aid in the diagnosis of a wide rangeRead MoreCalcium Hardness And Shell Thickness1070 Words   |  5 Pagesresults have already been confirmed by Marks and Washburn (1991) reports. Blood parameters The effects of dietary AA and LAB supplementation on serum parameters are summarized in Table 3. Serum glucose, ALT and triglyceride were decreased by feeding AA or LAB but not significant by the combination of AA and LAB. However, serum total cholesterol was decreased in all supplementation groups and synergistically by the combination of AA and LAB. Serum AST and ALP were not influenced by probiotics feedingRead MoreSalmonella Bacteria For Producing Protein Of Interest And Rate Growth And Protein Concentration After Inoculate The E.coli Culture1137 Words   |  5 PagesGOALS The goals of this lab report are to induce E.coli bacteria for producing protein of interest and determined the rate growth and protein concentration after inoculate the E.coli culture. In addition, protein visualizing and analyzing the success of IPTG induction, protein solubility and affinity chromatography demonstrated using SDS-PAGE. This report will also highlight the methodology of affinity chromatography that used to purify fused protein with poly His tag. A comparison between the advantageRead MoreIntroduction:. Cortisol Is A Steroid Hormone, Found In1609 Words   |  7 Pagesinfluence of a great number of variables remains to be determined† (Michaud et al. 2008). A report, written by Matheson and Cole (as cited in Michaud et al. 2008) stated that threats to an individual’s group were related to the increase in cortisol levels, particularly among individuals who had the tendency to express higher levels of anger. This experiment involved the use an of ELISA kit which stands for Enzyme- Linked- ImmunoSorbent Assay. This is based on the principle of competitive binding. A mouseRead MoreThe Mechanisms Linking Ribosomal Translation And Pre Mrna Processing10128 Words   |  41 Pages Investigation of the mechanisms linking ribosomal translation and pre-mRNA processing in yeast Nine-Month Report submitted by Marija Petrić ID: 1454106 Supervisor: Dr Saverio Brogna Contents Contents 1. Introduction 3 1.1. Eukaryotic gene expression 3 1.2. Quality control of eukaryotic gene expression 5 1.3. Nonsense mediated mRNA decay 6 1.4. Factors involved in NMD 7 1.5. NMD models 17 1.6. Ribosome release model 21 1.7. Role of UPF1 in the nucleus 23 2. Objectives 25 3. Materials and methodsRead MoreAn Introduction to Hydrophonics and Controlled Environment Agriculture40110 Words   |  161 Pagespavilions† oriented toward the south to grow flowers (though mainly for the wealthy to enjoy!). *1500’s and 1600’s – The expanding glass industry in Italy probably prompted Italians, English, Germans and French to experiment with glass for plant growing: reports of glass bells to cover plants, greenhouses used to grow flowers in the winter using solar heating, the building of â€Å"orangeries† for growing oranges and other citrus (to help guard against scurvy during the winter) and the first greenhouse or conservatory